Week 15 (01/23/23 - 01/27/23) Final MIC testing on D. aquaticus
Introduction: Before performing a procedure like transformation for a specific organism, it is essential to know what antibiotic that organism responds to, and the concentration of that antibiotic needed. When working with D. aquaticus , it was determined last semester that kanamycin is the best antibiotic to use to inhibit bacterial growth. Even though kanamycin is the ideal choice, we run into an issue when it comes to the plasmids that will be used in transformation. I believe we are working with the plasmid pwtCas9, which has an ampicillin resistance gene and a tetracycline resistance gene. Plasmid map for pwtCas9 is shown in figure 1. Because it does not have a kanamycin resistance gene, it is best to move forward with an antibiotic that the plasmid contains a resistance gene for. In this case, for our project we will be moving forward with tetracycline. Figure 1: Plasmid map for pwtCas9. The minimum inhibitory concentration (MIC) is the minimum amount of an...