S-STEM Scholar Jessica Sabel's Blog

  Introduction:   Kanamycin is a protein synthesis inhibitor that binds to the 30S subunit of the ribosome. It is an aminoglycoside that typically treats gram negative bacteria. Based on the Minimum Inhibitory Concentration (MIC) run from 9/14-9/16, kanamycin was chosen as the antibiotic of choice for  D. aquaticus ’ transformation. An additional MIC test was run from 9/21-9/23, with results showing that the concentration of Kanamycin is somewhere around 1.56 ug/ml. Because the results were not significant (mean absorbance was 0.27), additional MIC testing is required to find a more exact concentration of kanamycin to be used in the transformation of  D. aquaticus . Plasmid extractions were done to help the BIO208 class. The pUC18 plasmid specifically is the plasmid that will be targeted during the extraction. This plasmid is specific to  E. coli.     Methods:     MINIMUM INHIBITORY CONCENTRATION   D. aquaticus  has been inoculated ...

Introduction: All antibiotics belong to one of the five mechanisms of action: cell wall synthesis inhibitor, protein synthesis inhibitor, nucleic acid synthesis inhibitor, cell membrane disruption, and cellular respiration inhibitor. The antibiotic of choice for Deinococcus aquaticus , kanamycin, is a protein synthesis inhibitor that belongs to the antibiotic class called the aminoglycosides. This antibiotic binds to the 30S subunit of the ribosome, causing misalignment between the ribosome and the messenger RNA. This leads to the mRNA being misread and incorrect amino acid production, making it impossible for proteins to be synthesized. Kanamycin typically treats gram-negative bacteria, with the main bacteria of target being Mycobacterium tuberculosis . The Minimum Inhibitory Concentration (MIC) test run on 09/15 was performed using 3 different antibiotics: kanamycin, tetracycline, and chloramphenicol. Kanamycin and chloramphenicol were tested with the same concentrations of antibioti...